Some apoptotic bodies (Figure 1A). Apoptotic bodies are approximately in the size range of platelets (1-4 am), whereas micro particles are much smaller (< 1 am). Annexin V/FITC staining showed that both apoptotic bodies and micro particles are positive for annexing V (Figure 1B). In contrast, staining with PI showed that apoptotic bodies, but not micro particles, are positive for PI (Figure 1B). Furthermore, apoptotic bodies stained positive for DAPI, PI, and lection, as demonstrated by RevTest fluorescence microscopy (Figure 1C). These findings demonstrate that EC-derived apoptotic bodies exist as small membrane vesicles, which contain DNA. Circulating EPCs play a role in the repair of injured vessels and ischemic or damaged tissue.10 because endothelial injury is often associated with apoptosis, we have investigated whether apoptotic bodies from mature ECs could affect the behavior of adult EPCs in vitro. We demonstrated here a stimulatory effect of HUVEC-derived apoptotic bodies on the number and.
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